A number of our customers have asked for a protocol which would enable them to use the NEXTflex™ Small RNA Seq Kit v3 for ribosomal profiling, RIP-Seq (RNA binding protein immunoprecipitation and sequencing), and CLIP-Seq (cross-linking immunoprecipitation and sequencing) experiments. To use the NEXTflex Small RNA-Seq Kit V3, the RNA of interest must have a 5′ monophosphate and 3′ hydroxyl. Using the following steps posted on our website, these modifications can easily be added to most RNA molecules with T4 polynucleotide kinase (PNK).