One of the biggest drawbacks of the Roche NimbleGen SeqCap EZ Library target enrichment system is that only 24 barcodes and blockers are available for multiplexing. To increase multiplexing capabilities, Bioo Scientific has incorporated up to 96 ready-to-use barcodes into the NEXTflex™ Pre-Capture Combo Kit, allowing samples to be pooled prior to target capture, reducing cost and offering greatly increased throughput for Illumina-compatible sequencing. Using the NEXTflex Pre-Capture Combo Kit, researchers can expect comprehensive coverage of coding regions, high target enrichment efficiency and maximized variant discovery which has been validated by both Bioo Scientific and Roche NimbleGen.

[slideshare id=47519080&doc=bioo-scientific-increase-your-multiplexing-capacity-for-seqcap-target-capture-150428103701-conversion-gate02]