Bioo Scientific’s NEXTflex™ Pre-Capture Combo Kit is designed with up to 96 barcodes and blockers for unparalleled degrees of multiplexing both upstream and downstream of target capture. This kit from Bioo Scientific was developed to work in combination with the Roche NimbleGen SeqCap EZ Library capture systems for targeted exome sequencing on the Illumina sequencing platforms.

The NEXTflex Pre-Capture Combo Kit allows up to 96 samples to be pooled prior to target capture, reducing cost and offering incomparable throughput. Using this library kit, optimized for subsequent target enrichment, the user can expect more comprehensive coverage of coding regions, higher target enrichment efficiency and maximized variant discovery. In addition to the 96 unique barcodes, Bioo Scientific provides ready-to-use NEXTflex™ DNA Barcode Blockers for target capture.

All of the NEXTflex™ kits incorporate proprietary Enhanced Adapter Ligation Technology, which offers the highest ligation efficiency available, resulting in longer and more diverse sequencing reads. Bioo Scientific’s robust enzymes ensure the highest quality libraries for superior target enrichment and performance using the Nimblegen SeqCap EZ Library capture systems.

Our recent white paper written in conjunction with Roche NimbleGen demonstrates the efficacy of this kit when used with the NimbleGen SeqCap EZ Library system for library prep for Illumina-compatible sequencing.

Read more here.