Drs. Wessels, Hirsekorn, Ohler and Mukherjee from the Max Delbrück Center for Molecular Medicine recently published a RIP-Seq protocol which can be used to uncover genome-wide RNA transcripts that interact with a specific protein or protein complex. In this assay, ribonucleoprotein (RNP) complexes are immunoprecipitated (RIP) from cell lysates. Associated RNAs are then isolated from these RNP complexes. After the associated RNAs were isolated, these researchers used the NEXTflex™ Rapid Directional qRNA-Seq™ Kit for library preparation. Resulting libraries are suitable for sequencing on any Illumina sequencing platform.

Read the featured publication here: http://www.biooscientific.com/Identifying-RBP-Targets-with-RIP-Seq

Wessels, H.-H., Hirsekorn, A., Ohler, U. and Mukherjee, N. (2015) Identifying RBP Targets with RIP-seq. Post-Transcriptional Gene Regulation. Methods in Molecular Biology. 1358. 141-152.